陆地棉花发育EST测序分析及其相关基因筛选与微卫星标记开发

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陆地棉是世界上重要的经济作物之一,由于没有基因组数据,大量表达序列标签数据已被获得并广泛应用到各个研究领域当中。然而,在陆地棉花发育过程中,EST的信息很少。为了给陆地棉花发育分子机理的研究提供序列基础,本文对以中棉所36茎尖、蕾和花混合RNA为材料而构建的全长均一化cDNA文库进行了大规模测序,获得了22915条ESTs,经过组装后获得4563条contigs和9810条singletons,一共14373条unigeneso将这些获得的序列与公共数据库中已有的棉花EST序列进行比较分析发现,约有
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