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ＡＴ５ＢＩＶＡ细胞是一株经ＳＶ４０病毒转化的ＡＴ病人皮肤成纤维细胞，对γ射线高度敏感。实验用ＦＤ３（含人第１１号染色体的人鼠杂种细胞）、ＦＤ８（不含人第１１号染色体的人鼠杂种细胞）、ＬＭ／ＴＫ鼠细胞）为洪体，通过微细胞介导染色体转移（ＭＭＣＴ）向ＨＴ５ＢＩＶＡ细胞导入人或鼠的完整染色体，经两次３Ｇｙγ射线照射筛选后，获得ＡＴ５ＢＩＶＡ与ＦＤ３微细胞融合的杂合细胞ＡＴ／ＦＤ３－１，对γ射线抗性有显著提高。而ＦＤ８或ＬＭ／ＴＫ的微细胞与ＡＴ５ＢＩＶＡ细胞的杂合细胞，对γ射线抗性未增加。枝型分析表明ＡＴ／ＦＤ３—１细胞中包含了来源于ＦＤ３细胞的人第１１，１４号染色体和数条鼠染色体。通过对照实验，排除了人１４号和鼠染色体提高ＡＴ／ＦＤ３－１细胞对γ射线抗性的可能性．确认人第１１号染色体与ＡＴ细胞对电离辐射敏感性相关，提示人第１１号染色体上可能存在决定细胞对γ射线抗性的相关基因。
AT5BIVA cells are an AT patient’s skin fibroblasts transformed with SV40 virus and are highly sensitive to gamma rays. Experimental FD3 (human and mouse hybrid cells containing human chromosome 11), FD8 (human and mouse hybrid cell without human chromosome 11), LM / TK mouse cells) were isolated and cultured by microscopic cell-mediated chromosome transfer (MMCT) into HT5BIVA cells into human or mouse intact chromosomes, after two 3Gy γ-ray irradiation screening AT5BIVA and FD3 microcell fusion of hybrid cells AT / FD3-1, γ-ray resistance was significantly improved. While hybrid cells of FD8 or LM / TK minicells and AT5BIVA cells showed no resistance to gamma irradiation. Branching analysis showed that AT / FD3-1 cells contained human chromosomes 11 and 14 and several murine chromosomes derived from FD3 cells. By control experiments, the possibility that human chromosome 14 and murine chromosomes increase the gamma-ray resistance of AT / FD3-1 cells was excluded. Confirmation that human chromosome 11 is associated with AT cell sensitivity to ionizing radiation suggests that there may be genes that determine the resistance of cells to gamma radiation on chromosome 11.