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[目的]研究甘正复方对大鼠非酒精性脂肪肝模型形成的影响。[方法]30只SD大鼠随机分为3组(各10只):正常组喂普通饲料;模型组和治疗组喂高脂饲料。治疗组高脂饮食12周后同时给予甘正复方治疗。16周处死大鼠。测定血清丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、总胆固醇(TC)、三酰甘油(TG)、丙二醛(MDA)、超氧化物歧化酶(SOD)活性;苏木精-伊红染色观察肝脏病理改变;免疫组化法测定细胞色素P450ⅡE1(CYPⅡE1)及过氧化物酶体增殖物激活受体α(PPARα)表达。[结果]模型组血清ALT、AST、TC、TG及MDA增加,SOD减少;免疫组化示CYPⅡE1表达增高,PPARα表达明显减少;肝脏组织出现脂肪变性和炎症坏死。治疗组较模型组血清ALT、AST及TG、MDA均下降,SOD增加,CYPⅡE1表达减少,肝脏脂肪变性和炎症坏死的程度明显减轻。[结论]甘正复方能通过调节TG代谢、抗氧应激和脂质过氧化有效地治疗大鼠脂肪肝。
[Objective] To study the effect of Ganzheng compound on nonalcoholic fatty liver model formation in rats. [Methods] Thirty SD rats were randomly divided into 3 groups (10 each): the normal group was fed normal feed; the model group and the treatment group were fed high-fat diet. The treatment group was given Ganzheng compound after 12 weeks of high-fat diet. Rats were sacrificed at 16 weeks. Determination of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), triglyceride (TG), malondialdehyde (MDA), superoxide dismutase (SOD) Activity; Hematoxylin-eosin staining was used to observe the pathological changes of the liver; immunohistochemistry was used to measure the expression of cytochrome P450IIE1 (CYPIIE1) and peroxisome proliferator-activated receptor α (PPARα). [Results] Serum ALT, AST, TC, TG and MDA in model group increased, and SOD decreased. Immunohistochemistry showed that the expression of CYPIIE1 was increased, and PPARα expression was significantly reduced; liver tissue showed steatosis and inflammation necrosis. Compared with the model group, serum ALT, AST, TG, and MDA levels in the treatment group decreased, SOD increased, CYPIIE1 expression decreased, and the degree of hepatic steatosis and inflammation necrosis was significantly reduced. [Conclusion] Ganzheng compound can effectively treat rat fatty liver by regulating TG metabolism, anti-oxidation stress and lipid peroxidation.