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目的:研究脂多糖(LPS)对星形胶质细胞(AC)生长的影响及其可能的机制。方法:在原代培养的AC中加入不同浓度的LPS作用不同时间,观察AC生长的情况,以及NF-κB通路抑制剂SN50对AC生长的影响。同时以不同浓度的LPS作用24h后,观察随后8dAC生长的变化。结果:在LPS作用不同时间中,只有作用24h后,低剂量的LPS可使AC的生长加快,高剂量的LPS则可抑制AC生长。以LPS作用AC24h后,短期内LPS可促进AC的生长,长期则抑制AC生长,且呈剂量依赖性。NF-κB通路抑制剂可阻断LPS对AC生长的影响。结论:低剂量的LPS短期内可促进AC生长,高剂量时则抑制AC生长,而炎症对AC的长期影响是对细胞的生长增殖起抑制作用。其可能的分子机制可能与NF-κB通路激活有关。
Objective: To study the effect of lipopolysaccharide (LPS) on the growth of astrocytes (AC) and its possible mechanism. Methods: Different concentrations of LPS were added into primary culture AC for different time to observe the growth of AC and the effect of NF-κB pathway inhibitor SN50 on the growth of AC. At the same time, the effect of different concentrations of LPS for 24 h was observed after 8dAC growth. RESULTS: LPS at a low dose could accelerate the growth of AC and LPS at a higher dose could inhibit the growth of AC at different time of LPS. After treated with LPS for 24 hours, LPS could promote the growth of AC in a short term, and inhibit the growth of AC in the long term in a dose-dependent manner. NF-κB pathway inhibitors can block the impact of LPS on AC growth. CONCLUSION: LPS at low dose can promote the growth of AC in short term and inhibit the growth of AC at high dose. The long-term effect of inflammation on AC is inhibitory effect on the growth and proliferation of cells. Its possible molecular mechanism may be related to NF-κB pathway activation.