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目的 研究血小板对单核细胞促凝活性的影响。方法 采用一期法血浆凝固时间测定单核细胞促凝活性 ;单抗中和法确定组织因子 (TF)活性及P 选择素的作用。结果 ①内毒素激活的单核细胞促凝活性显著增高 ,TF单抗能明显阻断该效应 ;②胶原激活的血小板对单核细胞及内毒素激活的单核细胞的促凝活性均有明显的增强作用 ;③抗血小板P 选择素单抗能阻断活化的血小板对单核细胞促凝活性的增强效应。结论 ①单核细胞促凝活性是由于TF的表达 ;②血小板对单核细胞促凝活性的增强效应是通过P 选择素实现的。
Objective To study the effect of platelet on procoagulant activity of monocytes. Methods Monocyte procoagulant activity was determined by the method of primary plasma coagulation. The activity of tissue factor (TF) and the effect of P-selectin were determined by the monoclonal antibody neutralization method. Results ① The procoagulant activity of endotoxin-activated monocytes was significantly increased, and the effect of TF monoclonal antibody was obviously blocked. ② The procoagulant activity of collagen-activated platelets on monocytes and endotoxin-activated monocytes were significantly Enhance the role; ③ anti-platelet P-selectin monoclonal antibody can block the activated platelet monocyte procoagulant activity enhancement effect. Conclusion ① The procoagulant activity of monocytes is due to the expression of TF; ② The enhancing effect of platelets on the procoagulant activity of monocytes is achieved through P-selectin.