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目的前期的研究证实,染色体驱动蛋白KIF4A在胃癌组织中低表达,过表达KIF4A抑制了胃癌细胞的增殖。本研究进一步研究KIF4A与胃癌细胞迁移与转移的关系,从而为胃癌临床治疗提供新的治疗靶点。方法应用组织芯片和免疫荧光染色技术研究胃癌及其周围淋巴结组织中KIF4A蛋白分子的表达差异性;利用细胞小分子RNA干扰和Transwell迁移实验,检测KIF4A对细胞迁移运动能力的影响。结果 65%(26/40)的胃癌组织中KIF4A蛋白分子表达水平低于周围的淋巴结组织;KIF4A的表达水平与胃癌组织的TNM分期密切相关;KIF4A蛋白分子的表达随胃癌转移侵袭能力的增强而降低;抑制胃癌细胞内KIF4A蛋白分子的表达明显增强胃癌细胞的迁移运动能力。结论染色体驱动蛋白分子KIF4A通过抑制胃癌细胞的迁移运动能力参与控制胃癌的临床转移过程。
Objective The previous study confirmed that KIF4A was lowly expressed in gastric cancer tissues and over-expressed KIF4A inhibited the proliferation of gastric cancer cells. This study further study the relationship between KIF4A and gastric cancer cell migration and metastasis, so as to provide a new therapeutic target for the clinical treatment of gastric cancer. Methods Tissue microarray and immunofluorescent staining were used to investigate the expression of KIF4A protein in gastric cancer and its peripheral lymph nodes. The effects of KIF4A on the migration and migration of cells were detected by RNA interference and Transwell migration assay. Results The expression of KIF4A protein in 65% (26/40) gastric cancer tissues was lower than that in the surrounding lymph node tissues. The expression level of KIF4A was closely related to the TNM stage in gastric cancer tissues. The expression of KIF4A protein increased with the invasion and metastasis of gastric cancer Reduce the expression of KIF4A protein in gastric cancer cells significantly enhance the ability of gastric cancer cells to migrate. Conclusions KIF4A is involved in controlling the clinical metastasis of gastric cancer by inhibiting the ability of gastric cancer cells to migrate.