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目的探讨胃镜活检标本中早期胃癌与高级别上皮内瘤变及胃炎TRIM29、SKP2、MLH1抗体表达的差异性,寻找早期胃癌与高级别上皮内瘤变的诊断鉴别方法。方法使用免疫组织化学方法对46例胃炎标本、44例低级别上皮内瘤变组和42例高级别上皮内瘤变胃镜活检标本进行染色观察,采用统计学分析,比较其表达的差异性。结果 40例胃炎组中TRIM29无表达,SKP2弱表达,MLH1有3例表达。44例低级别上皮内瘤变组中MLH1全部表达,TRIM29有18例表达,SKP2全部表达。42例早期高级别上皮内瘤变中TRIM29全部表达,SKP2有28例表达,MLH1全部表达。三种抗体在3组间的表达比较差异有统计学意义,且表达程度与胃癌恶性进程有明显相关性。结论 TRIM29、SKP2、MLHI三种抗体可以作为胃镜活检标本中胃炎与上皮内瘤变及早期胃癌诊断的可靠标记物。
Objective To investigate the difference of expression of TRIM29, SKP2 and MLH1 between early gastric cancer, high-grade intraepithelial neoplasia and gastritis in gastroscopic biopsy specimens and to find out the differential diagnosis of early gastric cancer and high-grade intraepithelial neoplasia. Methods 46 cases of gastritis, 44 cases of low grade intraepithelial neoplasia and 42 cases of high grade intraepithelial neoplasia were examined by immunohistochemical staining. The difference of expression was compared by statistical analysis. Results 40 cases of gastritis group TRIM29 no expression, SKP2 weak expression, MLH1 3 cases. In 44 cases of low grade intraepithelial neoplasia, MLH1 was all expressed, 18 cases of TRIM29 and all of SKP2. TRIM29 was all expressed in 42 cases of early high grade intraepithelial neoplasia, with 28 cases of SKP2 and all of MLH1. The expression of the three antibodies in the three groups was significantly different, and the expression level was significantly correlated with the malignant progression of gastric cancer. Conclusion The three TRIM29, SKP2 and MLHI antibodies can be used as a reliable marker for the diagnosis of gastritis, intraepithelial neoplasia and early gastric cancer in gastroscope biopsy specimens.