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目的:研究ghrelin对大鼠晚期败血症休克的防治作用。方法:用盲肠结扎并穿刺法制备大鼠败血症休克模型,在术后即刻和8小时分别从股静脉和皮下注射ghrelin 10 nmol/kg。术后18h测定大鼠的血流动力学指标、血浆ghrelin、乳酸、葡萄糖浓度及心肌ATP含量。结果:与败血症休克组比较,ghrelin治疗组大鼠血压升高了33％,+LVdp/dt_(max)和-LVdp/dt_(max)值分别增加了27％和33％,而LVEDP降低了33％,血糖水平升高了54％,血浆乳酸浓度降低了40％,心肌ATP含量增加了22％(P<0.01)。死亡率由44％降到25％。败血症休克大鼠血浆ghrelin水平比假手术组(149±23)pmol/L增加了51％(P<0.01),血浆ghrelin水平与动脉血压和血糖值呈显著负相关(相关系数分别为-0.721和-0.811,P<0.01)。结论:ghrelin治疗可以部分纠正败血症休克大鼠的血流动力学紊乱和代谢障碍。
Objective: To study the preventive and therapeutic effects of ghrelin on shock in rats with advanced sepsis. Methods: The model of septic shock was prepared by cecal ligation and puncture. Ghrelin 10 nmol / kg was injected subcutaneously immediately and 8 h after operation. The indexes of hemodynamics, plasma ghrelin, lactate, glucose concentration and myocardial ATP content were measured 18h after operation. Results: Compared with septic shock group, the blood pressure of ghrelin treatment group increased by 33%, + LVdp / dt max and -LVdp / dt max increased by 27% and 33% respectively, while LVEDP decreased by 33% %, Blood glucose level increased by 54%, plasma lactate concentration decreased by 40%, myocardial ATP content increased by 22% (P <0.01). The death rate dropped from 44% to 25%. Plasma ghrelin levels in septic shock rats increased by 51% (P <0.01) compared with those in sham operation group (149 ± 23) pmol / L and plasma ghrelin levels were negatively correlated with arterial blood pressure and blood glucose (correlation coefficients were -0.721 and -0.811, P <0.01). Conclusion: ghrelin treatment can partially correct hemodynamic disorders and metabolic disorders in septic shock rats.