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目的:探讨胰腺癌组织中p53蛋白、胰岛素生长因子受体(IGP-r)的表达及临床意义。方法:应用抗生蛋白链菌素-生物素标记法(LSAB)检测39例胰腺癌和19例慢性胰腺炎组织中p53蛋白和IGF-r的表达,并对胰腺癌和慢性胰腺炎及不同分化程度胰腺癌组织中p53蛋白和IGF-r的阳性表达率进行比较。结果:(1)p53蛋白、IGF-r的表达产物主要定位于细胞核或细胞浆中,细胞分布不均匀;(2)胰腺癌组织中p53蛋白、IGF-r的阳性表达率分别为61.5%、66.7%,明显高于慢性胰腺炎组织(5.2%、36.8%)(P<0.05);(3)高分化胰腺癌中p53蛋白、IGF-r的阳性表达率分别为20.0%、33.3%,明显低于中、低分化胰腺癌(81.8%-92.3%、76.9%-81.8%)(P< 0.05)。结论:(1)p53抑癌基因突变与IGF-r的异常表达在胰腺癌的发生、发展过程中具有重要作用;(2)p53蛋白、IGF-r的过度表达与癌组织的分化程度有关;(3)p53蛋白与IGF-r可以作为鉴别胰腺良、恶性病变和进行诊断及判断预后的分子指标。
Objective: To investigate the expression of p53 protein and insulin-like growth factor receptor (IGP-r) in pancreatic cancer and its clinical significance. Methods: The expressions of p53 protein and IGF-r in 39 cases of pancreatic cancer and 19 cases of chronic pancreatitis were detected by streptavidin-biotin-labeled method (LSAB). The expressions of p53 protein and IGF-r in pancreatic cancer and chronic pancreatitis and their differentiation degree The positive rates of p53 protein and IGF-r in pancreatic cancer tissues were compared. Results: (1) The expression of p53 protein and IGF-r mainly localized in the nucleus or cytoplasm, and the cells were unevenly distributed. (2) The positive rates of p53 and IGF-r in pancreatic cancer tissues were 61.5% 66.7%, significantly higher than those in chronic pancreatitis (5.2%, 36.8%) (P <0.05). (3) The positive rates of p53 and IGF-r in well-differentiated pancreatic cancer were 20.0% and 33.3% Lower than that of moderate and poorly differentiated pancreatic cancer (81.8% -92.3%, 76.9% -81.8%) (P <0.05). Conclusions: (1) Mutation of p53 tumor suppressor gene and abnormal expression of IGF-r play an important role in the occurrence and development of pancreatic cancer; (2) The overexpression of p53 protein and IGF-r is related to the differentiation of cancer tissue; (3) p53 protein and IGF-r can be used as a molecular index to differentiate benign and malignant pancreatic lesions and to diagnose and prognose prognosis.