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The single-layer porous silicon is prepared by electrochemistry etching method,which is used as an immunosensor for determining recombinant mouse zona pellucida 3 fusion protein(r-mZP3) by Raman spectroscopy analysis at room temperature.The molecule binding increases the effective optical thickness(EOT),and thus the Raman spectrum intensity decreases.The concentration and variation of Raman intensity show a good linear quantitative relation.The excellent sensing performance could open the way to a new family of optical sensors for biological standardization.
The single-layer porous silicon is prepared by electrochemistry etching method, which is used as an immunosensor for determining recombinant mouse zona pellucida 3 fusion protein (r-mZP3) by Raman spectroscopy analysis at room temperature. The molecule binding increases the effective optical thickness ( EOT), and thus the Raman spectral intensity decreases. The concentration and variation of Raman intensity show a good linear quantitative relation. Excellent sensing performance could open the way to a new family of optical sensors for biological standardization.