目的　探讨混合酸脱钙时间及脱钙温度对切片质量的影响。方法　通过脱钙 ,记录脱钙时间、温度 ,制作石蜡切片 ,HE染色 ,显微镜观察等步骤 ,比较混合酸的脱钙时间及脱钙温度对切片质量造成的影响。结果　室温越高 ,脱钙时间越短 ,染色效果越好。结论　室温时 ,最佳脱钙时间为 8～ 9d ,选择合适的混合酸 ,保持其浓度能取得更好的石蜡切片 Objective To investigate the effects of mixed acid decalcification time and decalcification temperature on the quality of sections. Methods The decalcification time, temperature, paraffin section, HE staining and microscopic observation were used to compare the effects of decalcification time and decalcification temperature on slice quality. The results of the higher room temperature, decalcification time is shorter, the better the staining effect. Conclusion At room temperature, the best decalcification time is 8 ~ 9d, choose the appropriate mixed acid, to maintain its concentration can get better paraffin sections