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目的:研究Hodgkin提出的兴奋性分型是否存在于背根神经节(Dorsal Root Ganglion,DRG),并对该分型及介导该分型的离子通道电流的变化在慢性痛信号产生中的重要作用进行探讨。方法:本研究通过制备背根节慢性压迫模型(chronic compression of DRG,CCD),在整节消化后的DRG大中细胞进行全细胞膜片钳记录。结果:依照神经元受刺激后产生动作电位的特征,79只大鼠的311例神经元可分为三种类型。在正常对照组(n=179),1、2、3型神经元所占比例分别为13%、27%和60%;在CCD组(n=134)比例则分别为27%、33%和40%。统计分析显示慢性压迫损伤后,1型神经元分型比例明显增加,而3型神经元分型比例显著减少(P<0.05)。正常DRG神经元中,持续钠电流(INaP)的幅值在1、2型神经元中比3型神经元明显要高(P<0.05)。CCD术后INaP在1、2型神经元中均增加,但仅在1型神经元有显著性差异(P<0.05)。结论:DRG大中细胞在慢性压迫损伤后,神经元兴奋性分型中1、2型神经元的比例增多和1、2型神经元上INaP电流的增加,共同造成了由DRG向更高级中枢的信息传递的加强。
OBJECTIVE: To investigate whether Hodgkin’s excitatory typing is present in the dorsal root ganglion (DRG) and to study the importance of this typing and the changes in ion channel currents that mediate this typing in the development of chronic pain signals Role of the discussion. Methods: Whole-cell patch-clamp recording was performed in all DRG large and medium cells digested with chronic compression of DRG (CCD). Results: According to the characteristics of the action potential after the neurons were stimulated, 311 neurons of 79 rats can be divided into three types. In the normal control group (n = 179), the proportion of type 1,2,3 neurons was 13%, 27% and 60% respectively; in the CCD group (n = 134), they were 27% and 33% 40%. Statistical analysis showed that after chronic compression injury, the proportion of type 1 neurons increased significantly, while the proportion of type 3 neurons decreased significantly (P <0.05). In normal DRG neurons, the amplitude of continuous sodium current (INaP) was significantly higher in type 1 and 2 neurons than in type 3 neurons (P <0.05). INaP increased in type 1 and type 2 neurons after the operation of CCD, but there was significant difference only in type 1 neurons (P <0.05). CONCLUSION: After chronic compression injury of DRG large and medium cells, the proportion of type 1 and type 2 neurons in type 1 and type 2 neurons increases with the increase of INaP currents in neurons. The strengthening of information delivery.