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目的 :原位检测幽门螺杆菌 (Hp)感染时胃黏膜活检标本中肿瘤坏死因子 α(TNF α)和白介素 1 β(IL 1 β)的产生情况。方法 ;采集 1 3例Hp阴性受检者和 32例Hp阳性受检者的胃黏膜活检标本 ,通过放免法、免疫组织化学法观察并比较两组胃黏膜活检标本中细胞因子TNF α和IL 1 β的产生。结果 :胃黏膜活检标本中TNF α和IL 1 β的分泌在Hp阳性组显著高于Hp阴性组 (P <0 .0 1 ) ,但其分泌与胃黏膜的病理分型似无显著相关性 (P >0 .0 5)。TNF α和IL 1 β的组织表达在Hp阳性组也显著高于Hp阴性组 (P <0 .0 5)。结论 :Hp感染可以使局部细胞因子TNF α和IL 1 β产生增加 ,可能在Hp相关胃炎中起致病作用
Objective: To detect the production of tumor necrosis factor α (TNF α) and interleukin 1 β (IL 1 β) in gastric mucosa biopsy specimens in situ during Helicobacter pylori (Hp) infection. Methods Gastric mucosal biopsy specimens of 13 Hp-negative subjects and 32 Hp-positive subjects were collected. The expressions of cytokine TNFα and IL-1 in gastric mucosal biopsies were observed by immunohistochemistry and radioimmunoassay β production. Results: The secretion of TNF α and IL 1 β in gastric mucosa biopsy specimens was significantly higher in Hp positive group than in Hp negative group (P <0.01), but no significant correlation was found between the secretion of TNFα and IL 1 β in gastric mucosa P> 0 .0 5). The expression of TNFα and IL 1 β in Hp positive group was also significantly higher than that in Hp negative group (P <0.05). Conclusion: Hp infection can increase the production of local cytokines TNFα and ILβ and may play a pathogenic role in Hp-related gastritis