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水稻细菌性条斑病菌(Xamthomonas.oryzae pv.oryzicola,Xoc)是水稻的主要病原菌之一,其引发的水稻细菌性条斑病可造成水稻严重减产。本文利用水稻细菌性条斑病菌广西分离株GX01作为实验菌株,获得XOC2233基因的Tn5插入突变体,XOC2233编码为假定的甲基受体趋化性蛋白,本实验对其生化表型以及致病性的研究表明,突变体的致病力与野生型相比没有明显变化,但其胞外多糖产量和游动性增强,且其胞外蛋白酶的活性有所降低,而互补菌株均能恢复其表型到野生型水平。根据本研究的结果推测XOC2233基因可能通过对鞭毛的合成控制影响胞外多糖的分泌,为进一步研究XOC2233基因在水稻条斑病菌胞外多糖代谢途径中的作用提供了基础。
Xam is one of the major pathogenic bacteria in rice, and the rice bacterial leaf streak caused by it can cause serious rice yield loss. In this paper, GX01 was used as the experimental strain to obtain the Tn5 insertion mutant of XOC2233 gene, and XOC2233 was encoded as the hypothetical methyl receptor chemotaxis protein. In this experiment, its biochemical phenotype and pathogenicity Showed that the pathogenicity of the mutants did not change significantly compared with the wild type, but the extracellular polysaccharide production and mobility increased, and the extracellular protease activity decreased, while the complementary strains were able to restore the table Type to wild type. Based on the results of this study, it is speculated that XOC2233 gene may affect the secretion of extracellular polysaccharides by controlling the synthesis of flagella, which provides a basis for further study on the role of XOC2233 gene in the extracellular polysaccharide metabolic pathway of the pathogen.