论文部分内容阅读
目的探讨细胞色素P450酶(cytochromeP4501B1,CYP1B1)及儿茶酚-O-甲基转移酶(catechol-O-methytransferase,COMT)在子宫内膜癌发病机制中的作用。方法采用Western Blot技术检测2006-2008年河北医科大学第四医院子宫内膜癌组织39例(病例组)及正常子宫内膜组织22例(对照组)中CYP1B1及COMT蛋白的表达,并对两者进行比较。结果病例组中CYP1B1蛋白的表达量(3.637±1.697)明显高于对照组(2.371±1.145)(P=0.003);而S-COMT、M-COMT在病例组中的表达量(分别为1.104±0.576、0.970±0.461)明显低于对照组(分别为1.602±0.665,2.091±0.609)(P=0.001,P=0.000)。病例组CYP1B1/S-COMT及CYP1B1/M-COMT明显高于对照组(P=0.002,P=0.000)。三期子宫内膜癌中,CYP1B1及S-COMT的差异有统计学意义(P=0.000,P=0.001)。结论 CYP1B1与子宫内膜癌的发生发展有密切关系,可能成为子宫内膜癌治疗的新靶点及预后的指标。COMT在子宫内膜癌的发展中起到了保护作用。
Objective To investigate the role of cytochrome P450B1 (CYP1B1) and catechol-O-methyltransferase (COMT) in the pathogenesis of endometrial carcinoma. Methods Western Blot technique was used to detect the expression of CYP1B1 and COMT protein in 39 cases of endometrial carcinoma and 22 cases of normal endometrium from the Fourth Hospital of Hebei Medical University from 2006 to 2008, For comparison. Results The expression of CYP1B1 protein in case group was significantly higher than that in control group (3.637 ± 1.697 vs 2.371 ± 1.145, P = 0.003), while the expression of S-COMT and M-COMT in case group was 1.104 ± 0.576, 0.970 ± 0.461) was significantly lower than the control group (1.602 ± 0.665, 2.091 ± 0.609, respectively) (P = 0.001, P = 0.000). The cases of CYP1B1 / S-COMT and CYP1B1 / M-COMT were significantly higher than the control group (P = 0.002, P = 0.000). In stage III endometrial carcinoma, the differences of CYP1B1 and S-COMT were statistically significant (P = 0.000, P = 0.001). Conclusion CYP1B1 is closely related to the occurrence and development of endometrial carcinoma, which may be a new target for the treatment of endometrial carcinoma and an index of prognosis. COMT plays a protective role in the development of endometrial cancer.