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背景:细胞内过氧化物质增加产生的氧化损伤可以加速癫痫发作的频率,而通过氧化还原作用消耗过氧化物,可起到对中枢神经元的保护作用。目的:分析癫痫患者血浆及红细胞中抗氧化剂水平的变化。设计:非随机同期化的平行对照(相互对照、空白对照)。单位:一所大学医院的检验科、精神科、药剂科。对象:2000-03/12中南大学湘雅二医院神经内科门诊确诊的癫痫患者(癫痫疾病组)32例,均符合国际抗癫痫联盟的诊断及分类标准,且未经过任何治疗。男17例,女15例,年龄27～59岁。同期选择本院神经内科住院癫痫患者(治疗组)26例,符合国际抗癫痫联盟的诊断及分类标准,接受了1年多的苯巴比妥类药物的治疗,在此期间无癫痫急性发作的症状;男16例,女10例,年龄24～58岁。正常对照组为随机选取本院门诊体检人员39例,体检各项提标均在正常参考范围内;男23例,女16例。上述均为知情同意者。方法:3组对象于清晨8:00～9:00空腹采集静脉血2mL。采用酶偶联连续监测法测定红细胞的内谷胱甘肽还原酶,谷胱甘肽过氧化物酶,过氧化氢酶活性的测定。采用邻苯三酚自氧化比色法测定红细胞中超氧化物歧化酶活性。采用改良TBA比色法测定红细胞中丙二醛的含量。同时测定血红蛋白浓度和红细胞孵育渗透脆性(以溶血百分率计算)。采用高效液相色谱法测定血
Background: Oxidative damage caused by increased intracellular oxidants can accelerate the frequency of seizures, whereas peroxides depleted by oxidation and reduction can protect central neurons. Objective: To analyze the changes of antioxidants in plasma and erythrocytes of patients with epilepsy. Design: Parallel non-randomized parallel control (reciprocal control, blank control). Unit: a university hospital laboratory, psychiatry, pharmacy. PARTICIPANTS: 32 cases of epilepsy patients (epilepsy disease group) diagnosed by Department of Neurology, Second Xiangya Hospital of Central South University from March to December 2000 were in line with the diagnosis and classification criteria of the International Antiepileptic Association, without any treatment. 17 males and 15 females, aged 27 to 59 years. In the same period, 26 patients with in-hospital epilepsy in our department of neurology (treatment group) were selected, which met the criteria of diagnosis and classification of international antiepileptic alliance and received more than one year of treatment with phenobarbital. No acute seizures Symptoms; 16 males and 10 females, aged 24 to 58 years. The normal control group were randomly selected 39 outpatient medical staff in our hospital, physical examination of all the standard within the normal reference range; 23 males and 16 females. All of the above are informed consenters. Methods: Three groups of subjects were fasting venous blood 2mL at 8: 00-9: 00 in the morning. The activity of glutathione reductase, glutathione peroxidase and catalase in erythrocytes was determined by enzyme-linked continuous monitoring method. Determination of superoxide dismutase activity in erythrocytes by pyrogallol autoxidation colorimetry. The content of malondialdehyde in erythrocytes was determined by modified TBA colorimetry. Simultaneous determination of hemoglobin concentration and erythrocyte incubation osmotic fragility (calculated as% hemolysis). The blood was determined by high performance liquid chromatography