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目的:探讨抑癌基因p16在肝细胞癌发生、发展中的作用。方法:采用免疫组化及原位杂交方法在87例肝细胞癌及其癌旁肝组织内检测P16蛋白、HBxAg及p16mRNA。结果:在87例中,16例P16蛋白和19例p16mRNA阳性,分别占18.4%和21.8%。在53例癌旁肝组织中,32例P16蛋白和33例p16mRNA阳性,分别占60.4%和62.8%。在HBxAg阳性的70例中,9例P16蛋白阳性,HBxAg阴性的17例中,7例P16蛋白阳性。结论:肝细胞癌的发生可能与p16表达减少有关,HBVx基因表达产物可能参与p16基因表达的调控
Objective: To investigate the role of tumor suppressor gene p16 in the occurrence and development of hepatocellular carcinoma. Methods: P16 protein, HBxAg and p16 mRNA were detected in 87 cases of hepatocellular carcinoma and its surrounding liver tissue by immunohistochemistry and in situ hybridization. Results: In 87 cases, 16 cases of P16 protein and 19 cases of p16 mRNA were positive, accounting for 18.4% and 21.8%, respectively. In 53 cases of paracancerous liver tissues, 32 cases of P16 protein and 33 cases of p16 mRNA were positive, accounting for 60.4% and 62.8%, respectively. Of the 70 HBxAg-positive cases, 9 were positive for P16 protein and 17 were negative for HBxAg, and 7 were positive for P16 protein. Conclusion: The occurrence of hepatocellular carcinoma may be related to the decrease of p16 expression. The HBVx gene expression product may be involved in the regulation of p16 gene expression.