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目的：探讨抑癌基因ｐ１６在肝细胞癌发生、发展中的作用。方法：采用免疫组化及原位杂交方法在８７例肝细胞癌及其癌旁肝组织内检测Ｐ１６蛋白、ＨＢｘＡｇ及ｐ１６ｍＲＮＡ。结果：在８７例中，１６例Ｐ１６蛋白和１９例ｐ１６ｍＲＮＡ阳性，分别占１８．４％和２１．８％。在５３例癌旁肝组织中，３２例Ｐ１６蛋白和３３例ｐ１６ｍＲＮＡ阳性，分别占６０．４％和６２．８％。在ＨＢｘＡｇ阳性的７０例中，９例Ｐ１６蛋白阳性，ＨＢｘＡｇ阴性的１７例中，７例Ｐ１６蛋白阳性。结论：肝细胞癌的发生可能与ｐ１６表达减少有关，ＨＢＶｘ基因表达产物可能参与ｐ１６基因表达的调控
Objective: To investigate the role of tumor suppressor gene p16 in the occurrence and development of hepatocellular carcinoma. Methods: P16 protein, HBxAg and p16 mRNA were detected in 87 cases of hepatocellular carcinoma and its surrounding liver tissue by immunohistochemistry and in situ hybridization. Results: In 87 cases, 16 cases of P16 protein and 19 cases of p16 mRNA were positive, accounting for 18.4% and 21.8%, respectively. In 53 cases of paracancerous liver tissues, 32 cases of P16 protein and 33 cases of p16 mRNA were positive, accounting for 60.4% and 62.8%, respectively. Of the 70 HBxAg-positive cases, 9 were positive for P16 protein and 17 were negative for HBxAg, and 7 were positive for P16 protein. Conclusion: The occurrence of hepatocellular carcinoma may be related to the decrease of p16 expression. The HBVx gene expression product may be involved in the regulation of p16 gene expression.