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目的观察癫痫清颗粒对戊四唑诱发大鼠慢性癫痫模型的影响。方法大鼠腹腔注射10 g·L-1戊四唑(40 mg·kg-1),隔日1次,连续4周,停药l周后,再次注射相同剂量的戊四唑,根据癫痫发作级别随机分为7组每组10只,连续灌胃给药治疗四周,末次给药1 h后除空白对照组外各组大鼠腹腔注射10 g·L-1戊四唑(40 mg·kg-1),记录各组大鼠癫痫发作级别,并通过HE染色观察癫痫清颗粒对戊四唑诱发大鼠慢性癫痫模型海马组织结构的影响,并通过免疫组化染色及免疫印迹法观察癫痫清颗粒对戊四唑诱发大鼠慢性癫痫模型海马组织凋亡因子表达的影响。结果癫痫清颗粒组癫痫发作级别显著低于模型对照组,与模型对照组相比癫痫清颗粒可显著降低大鼠脑海马CA1区AIF和Ba X的表达,与模型对照组相比癫痫清颗粒可显著增加大鼠脑海马CA1区Bcl-X/S+L的表达。结论癫痫清颗粒对戊四唑诱发大鼠慢性癫痫模型具有呈剂量依赖性的保护作用。
Objective To observe the effect of epilepsy granules on senile pentylenetetrazole-induced chronic epilepsy in rats. Methods Rats were injected intraperitoneally with 10 g · L-1 penta tetrazolium (40 mg · kg-1) every other day for 4 weeks. After stopping for 1 week, the same dose of pentylenetetrazol was injected again. According to the level of seizure The rats in each group were randomly divided into 7 groups with 10 rats in each group. After continuous intragastric administration for 4 weeks, rats in the control group were injected intraperitoneally with 10 g · L-1 penta tetrazolium (40 mg · kg- 1) were used to record the epileptic seizure level in each group. The effect of epilepsy-clearing granule on the tissue structure of rat hippocampus induced by pentylenetetrazole-induced epilepsy was observed by HE staining. The expression of epilepsy clearance granule was observed by immunohistochemistry and immunoblotting Effect of pentylenetetrazol on the expression of apoptosis factor in hippocampus of rats with chronic epilepsy model. Results Epileptic seizures of epilepsy granules group was significantly lower than that of the model control group. Compared with the model control group, epilepsy granules can significantly reduce the expression of AIF and Ba X in hippocampal CA1 area of rats, compared with the model control group, epilepsy granules Significantly increased the expression of Bcl-X / S + L in hippocampal CA1 region of rats. Conclusion Epilepsia Granule has a dose-dependent protective effect on rats with chronic epilepsy induced by pentylenetetrazole.