论文部分内容阅读
利用BiologMT技术,研究了3个紫花苜蓿(Medicago sativa)品种(肇东苜蓿、中苜一号和阿尔冈金)的建植年限(两年和10年)对根际土壤微生物碳代谢多样性的影响。结果表明,建植10年紫花苜蓿的根际土壤在表征土壤微生物代谢活性的平均颜色变化率(AWCD)上明显高于建植两年的根际土壤,其中10年阿尔冈金的AWCD值最高,而两年肇东苜蓿的值最低。相同品种不同建植年限间,建植10年紫花苜蓿的根际土壤微生物McIntosh均匀度指数明显高于建植两年,而10年和两年根际土壤微生物在Shannon多样性指数和SimpsonMT优势度指数上差异不显著。这说明建植10年的紫花苜蓿改变了根际土壤微生物群落的组成结构,同时也提高了根际土壤微生物群落的多样性。通过主成分分析,相同品种、不同建植年限下土壤微生物的碳源利用模式出现的差异并不显著,但10年紫花苜蓿根际土壤微生物间碳源利用的模式存在差异,主要碳源为糖类、羧酸和氨基酸等。
BiologMT was used to study the effects of planting age (two years and ten years) of three Medicago sativa cultivars (Zhaodong alfalfa, Zhongmu No.1 and Algonquin) on microbial metabolism of rhizosphere soil influences. The results showed that the average color change rate (AWCD) of rhizosphere soils grown for 10 years on alfalfa was significantly higher than that of rhizosphere soils grown for two years, and the highest AWCD value of 10 years’ Algonquin , While the value of Zhaodong alfalfa was the lowest in two years. In the same variety and different planting years, the average microbial biomass McIntosh index in rhizosphere soil of 10-year-old alfalfa was significantly higher than that of the 2-year-old alfalfa. The soil microbial biomass at the 10-year and 2-year rhizosphere soil microbial biomass was significantly higher in the Shannon diversity index and Simpson MT dominance There is no significant difference in the index. This indicated that alfalfa 10 years after planting changed the composition and structure of rhizosphere soil microbial community and also increased the diversity of rhizosphere soil microbial community. Through principal component analysis, there was no significant difference in carbon source utilization patterns of soil microbes under the same variety and different planting years. However, there was a difference in the mode of carbon utilization among 10 years in rhizosphere soil of alfalfa, and the main carbon source was sugar Class, carboxylic acid and amino acid and so on.