目的:检测食管癌组织中线粒体DNA(mtDNA)的突变。方法:提取8例食管癌组织的mtDNA;PCR扩增mtDNA的控制区,纯化PCR产物、与T载体连接,转化大肠杆菌,筛选出阳性克隆,用引物T7/SP6做PCR鉴定后测序,与GenBank序列对比。结果:8例标本mtDNA的控制区共有36个位点发生突变,其中转换32个,颠换4个;同义突变13个,错义突变23个。结论:mtDNA突变很可能在食管癌的发生中发挥一定作用。 Objective: To detect the mutation of mitochondrial DNA (mtDNA) in esophageal cancer tissues. Methods: The mtDNA of 8 esophageal cancer tissues was extracted. The control region of mtDNA was amplified by PCR. The PCR product was purified and ligated with T vector. The recombinant plasmid was transformed into E.coli. The positive clones were screened out by PCR with primers T7 / SP6. Sequence comparison. Results: A total of 36 sites were mutated in the control region of 8 specimens, of which 32 were transposed and 4 were transposed; 13 were synonymous and 23 were missense mutations. Conclusion: mtDNA mutations may play a role in the occurrence of esophageal cancer.