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目的探讨姜黄素(Curcumin)对人喉癌Hep-2细胞的生长抑制及诱导凋亡作用。方法将Hep-2细胞用含不同浓度姜黄素(3、6、12.5、25μmol/L)的培养基分别培养24和48h,采用MTT法检测其对细胞增殖的影响;台盼蓝染色法检测其对细胞活力的影响;流式细胞术检测其对细胞周期分布及细胞凋亡的影响;DNA琼脂糖凝胶电泳检测其对细胞DNA的影响。结果姜黄素可明显抑制Hep-2细胞增殖,且呈时间-剂量依赖性;可明显降低细胞活力,且呈剂量依赖性;可使细胞阻滞在G2/M期,并诱导细胞出现典型的凋亡峰,凋亡率呈时间-剂量依赖性;能使细胞DNA形成典型的DNA-Ladder。结论姜黄素对人喉癌Hep-2细胞增殖及细胞活力具有显著的抑制作用,并能诱导Hep-2细胞发生凋亡。
Objective To investigate the effect of curcumin on growth inhibition and apoptosis of human laryngeal cancer Hep-2 cells. Methods Hep-2 cells were cultured with different concentrations of curcumin (3, 6, 12.5, and 25 μmol/L) for 24 and 48 h, respectively. MTT assay was used to detect the effect of Hep-2 cells on proliferation. The trypan blue staining method was used to detect Hep-2 cells. The effect on cell viability was examined by flow cytometry to determine cell cycle distribution and apoptosis. DNA agarose gel electrophoresis was used to examine its effect on cell DNA. Results Curcumin could significantly inhibit the proliferation of Hep-2 cells in a time- and dose-dependent manner. The activity of the cells was decreased in a dose-dependent manner. The cells were arrested in the G2/M phase, and the cells were induced to show typical withering. At the peak of death, the apoptotic rate was time- and dose-dependent; DNA DNA could form a typical DNA-Ladder. Conclusion Curcumin has a significant inhibitory effect on the proliferation and cell viability of human laryngeal carcinoma Hep-2 cells, and can induce apoptosis in Hep-2 cells.