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本文报告了用时间梯度确定大豆根尖的有丝分裂高峰期的方法。并用该法测定出省内外不同生态型栽培品种和不同进化类型(野生、半野生及栽培三种类型)的大豆材料都具有相同的有丝分裂高峰期。在贵阳地区,28℃培养条件下,其细胞分裂高峰期均在上午8时前后。 本试验的供试材料,在大豆的细胞周期中,共出现两次有丝分裂高峰(M_1为7:30～8:30; M_2为14:30～15:30)。其峰值,M_1比M_2高出近一倍。因而应用于染色体制片技术时,宜在M_1时间内进行大豆根尖细胞的前处理。
This paper reports a method to determine the mitotic peak of soybean apex using time gradient. The method was used to determine the peak of mitosis in different ecotype cultivars and different evolution types (wild, semi-wild and cultivated) of soybean materials both inside and outside the province. In Guiyang, under the culture conditions of 28 ℃, the peak of cell division was around 8:00 am. In this experiment, the mitosis peak appeared twice in the cell cycle of soybean (M_1 is 7:30 ~ 8:30; M_2 is 14:30 ~ 15:30). Its peak, M_1 almost higher than the M_2. Therefore, when applied to chromosome preparation technology, the root tip cells of soybean should be pretreated in M_1 time.