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采用巢式不平衡设计,按每尾雄虾配1～3尾雌虾进行人工授精构建家系。对其中13个家系进行生长和耐氨氮特性实验。8周的养殖实验结束后,各家系的特定生长率为2.97～4.00,饲料系数为1.04～1.74。其中家系S13-S11的特定生长率为4.00,明显优于其他家系;家系S3-S5的特定生长率在所有家系中最低,仅有2.97(P<0.05),且其饲料系数显著高于其他家系(P<0.05)。在生长实验结束后,进行氨氮胁迫实验。氨氮胁迫48 h后,不同家系的成活率存在差异性,S14-S15的成活率为98.33%,显著高于其他家系(P<0.05);血细胞数量与家系成活率呈正相关,SOD酶、AKP酶的活力与家系成活率呈负相关,POD酶的活力与家系成活率不具相关性。综合分析,以L2和S11为父本的半同胞家系生长快且抗氨氮特性强,可留作育种材料进行下一步选育。
Nested unbalanced design, according to each male with 1 to 3 male and female shrimp artificial insemination to build a family. Thirteen families were tested for growth and ammonia-tolerant properties. After 8 weeks of breeding experiment, the specific growth rate of each line was 2.97 ~ 4.00 and the feed coefficient was 1.04 ~ 1.74. Among them, the specific growth rate of family S13-S11 was 4.00, which was significantly better than other families. The specific growth rate of family S3-S5 was the lowest among all the families, only 2.97 (P <0.05), and its feed coefficient was significantly higher than other families (P <0.05). After the growth experiment was finished, ammonia nitrogen stress experiment was conducted. The survival rates of different families were different after ammonia nitrogen stress for 48 h, the survival rate of S14-S15 was 98.33%, which was significantly higher than that of other families (P <0.05). The number of blood cells was positively correlated with the survival rate of family members. SOD, AKP Of vitality and family survival rate was negatively correlated, POD enzyme activity and family survival rate is not related. According to the comprehensive analysis, the semi-sib families with L2 and S11 as the parents grow fast and have strong anti-ammonia nitrogen resistance, which can be used as breeding materials for further breeding.