论文部分内容阅读
目的比较经血宁胶囊5种提取部位之间凝血作用的差异,明确其凝血作用部位。方法对经血宁胶囊5种提取部位即石油醚部位(A)、乙酸乙酯部位(B)、正丁醇部位(C)、水溶部位(D)、残渣部位(E)进行凝血作用的药效筛选。(1)采用毛细玻管法和玻片法测定凝血时间(coagulation time,CT),观察经血宁胶囊5种提取部位对小鼠凝血时间的影响;(2)凝血酶原时间(prothrombin time,PT)测定;(3)活化部分凝血活酶时间(activated partial thromboplastin time,APTT)测定,探讨其凝血作用机制。结果 (1)毛细玻管法和玻片法测定小鼠凝血时间(重复3次),结果显示经血宁胶囊5种提取部位在9 g生药/kg剂量下均有不同程度的缩短凝血时间,其中正丁醇部位(C)重现性较好,提示正丁醇部位(C)具有较好的凝血作用。A、D、E提取部位凝血作用不明显。(2)经血宁胶囊5种提取部位的家兔PT时间分别与空白对照组比较,正丁醇部位(C)P<0.03,残渣部位(E)P<0.01,PTR值较小,提示C、E部位具有较好的缩短凝血酶原(PT)时间作用,其他部位影响凝血酶原时间不明显。(3)经血宁胶囊5种提取部位的血浆APTT分别与空白对照组比较,A、C、D、E部位均有显著性差异(P<0.01),提示均有具有较好的缩短APTT作用。结论经血宁胶囊正丁醇部位(C)在9 g生药/kg剂量下具有较好的缩短凝血时间的作用,且重复性较好,其他提取部位凝血作用不明显。
Objective To compare the difference of coagulation between 5 kinds of extracted parts of Jingxuening Capsule and clarify the site of coagulation. Methods The pharmacological effects of 5 kinds of extraction parts of Jingxuening capsule, namely petroleum ether fraction (A), ethyl acetate fraction (B), n-butanol fraction (C), water soluble fraction (D) and residual fraction filter. (1) Coagulation time (CT) was measured by capillary tube method and slide method to observe the influence of the five extraction sites of Jingxuening capsule on the clotting time of mice; (2) The prothrombin time (PT ); (3) activated partial thromboplastin time (APTT) determination to explore the mechanism of coagulation. Results (1) Coagulation time was determined by capillary tube method and slide method (repeated three times). The results showed that the five extraction sites of Jingxuening capsule had different degrees of shortening clotting time under 9 g crude drug / kg dose, of which, Butanol fraction (C) reproducibility is good, suggesting n-butanol (C) has a better coagulation. A, D, E extraction site coagulation is not obvious. (2) Compared with the blank control group, PT of the five extracts of Jingxuening capsule were significantly lower than those of the blank control group (P <0.03 for n-butanol fraction and P <0.01 for residue fraction), suggesting that C, E site has a better shortening prothrombin time (PT), other parts of the prothrombin time is not obvious. (3) Compared with the blank control group, there were significant differences in APTT (P <0.01) between the five extracts of Jingxuening capsule and the blank control group, suggesting that APTT has a good effect of shortening the APTT. Conclusion The n-butanol fraction of Jingxuening Capsule (C) has a good shortening clotting time at 9 g crude drug / kg dose with good repeatability and no significant coagulation in other extraction sites.