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利用TP-M13-SSR分子标记方法,构建27份中国原产苹果属植物在12个SSR位点的指纹图谱,并运用条码技术生成其分子身份证。12对引物共获得251个等位基因,平均21个。引物多态性好,仅用引物CH05b06即可区分全部供试材料。27份苹果材料在12个SSR位点遗传多样性、多态性信息含量和位点杂合度的变化范围分别为0.6620~0.9455、0.6327~0.9211和0.6538~0.9319。基于CH05b06位点处获得的指纹谱图即可得到每份供试材料独有的分子身份证。TP-M13-SSR分子标记技术适用于苹果属植物种质资源的指纹图谱构建,利于分子基础数据库的积累。基于苹果种质资源TP-M13-SSR指纹图谱可获得每份苹果种质资源独有的分子身份证。
The fingerprinting of 27 Chinese apple plants from 12 SSR loci was constructed using TP-M13-SSR molecular marker and its molecular ID was generated by using barcode technology. Twelve pairs of primers obtained a total of 251 alleles, an average of 21. Primer polymorphism is good, only primers CH05b06 can distinguish all test materials. The genetic diversity of 27 apple cultivars at 12 SSR loci varied from 0.6620 to 0.9455, from 0.6327 to 0.9211 and from 0.6538 to 0.9319 for the polymorphism information content and locus heterozygosity, respectively. Based on the fingerprinting obtained at CH05b06, a unique molecular ID can be obtained for each test material. The TP-M13-SSR molecular marker technique is suitable for the construction of fingerprinting of apple germplasm resources, which is beneficial to the accumulation of molecular basis database. Based on the apple germplasm TP-M13-SSR fingerprinting access to each apple germplasm unique molecular identity card.