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采用酶免疫(EIA)法,测定和计算人工麝香对环氧合酶Ⅱ(COX-2)的抑制率,构建人工麝香对COX-2抑制作用的量效关系,为建立人工麝香的体外抗炎效价测定方法提供研究基础。结果显示,人工麝香对COX-2具有明显抑制作用,半数抑制浓度(IC50)为2.26 mg·mL-1,在0.31～20.0 mg·mL-1内,具有明显的量效关系。结果表明,该方法快速、灵敏、准确、重现性好,可用于建立人工麝香抗炎生物效价检测方法,同时本文也为人工麝香的生物活性评价提供了新的研究思路和方法。
The enzyme-linked immunosorbent assay (EIA) was used to determine and determine the inhibition rate of artificial musk on cyclooxygenase Ⅱ (COX-2), and to establish a dose-response relationship of artificial musk on COX-2 inhibition. The titer method provides the basis for the study. The results showed that artificial musk had a significant inhibitory effect on COX-2 with IC50 of 2.26 mg · mL-1 and a significant dose-response relationship of 0.31-20.0 mg · mL-1. The results showed that the method was rapid, sensitive, accurate and reproducible, and could be used to establish a method for the detection of artificial musk anti-inflammatory biological potency. At the same time, this article provided a new research idea and method for the evaluation of biological activity of artificial musk.