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造血干/祖细胞移植已成为迄今为止治疗恶性血液病等最有效措施,而建立骨髓型可移植性白血病小鼠模型将为造血干细胞移植治疗白血病提供实验用动物模型。本实验用K562细胞接种BALB/c裸鼠产生红白血病的小鼠模型。将4-5周龄雌性BALB/c裸鼠,腹腔连续两天注射环磷酰胺(CTX)2mg,第3天腹腔或尾静脉直接接种K562白血病细胞2×105-2×106/只。定时取小鼠尾静脉外周血、骨髓细胞用流式细胞术和RT-PCR分别检测CD45,CD13,CD33抗原及bcr/abl融合基因。结果显示,4-5周龄BALB/c裸鼠无论通过腹部或尾静脉接种,无论有无CTX预处理,当接种K562细胞数大于2×105/只时,均可在BALB/c裸鼠身上产生可移植性人髓系白血病,荷瘤小鼠可存活30-60天。结论:腹腔或尾静脉接种大于2×105细胞/只均可产生人髓系白血病荷瘤小鼠模型,有无CTX2毫克/只的预处理不影响4-5周龄BALB/c裸鼠产生人白血病模型。
Hematopoietic stem / progenitor cell transplantation has become the most effective measure for the treatment of hematologic malignancies so far. However, the establishment of a mouse model of bone marrow-derived transplantable leukemia will provide an experimental animal model for the treatment of leukemia with hematopoietic stem cell transplantation. In this experiment, K562 cells inoculated BALB / c nude mice produce erythroleukemia mouse model. Female 4- to 5-week-old BALB / c nude mice were injected intraperitoneally with 2 mg of CTX twice a day for 2 consecutive days. K562 leukemia cells were inoculated 2 × 105-2 × 10 6 per day on the 3rd day. Peripheral blood of tail vein of mice was taken at regular intervals. CD45, CD13, CD33 antigen and bcr / abl fusion gene were detected by flow cytometry and RT-PCR respectively in bone marrow cells. The results showed that BALB / c nude mice aged 4-5 weeks, regardless of CTX pretreatment, were inoculated on the BALB / c nude mice either in the abdomen or in the caudal vein, when the number of inoculated K562 cells was more than 2 × 10 5 / Produce transplantable myeloid leukemia, tumor-bearing mice can survive 30-60 days. CONCLUSION: Human myeloid leukemia tumor-bearing mice can be induced by intraperitoneal or caudal vein inoculation of more than 2 × 10 5 cells / ml, respectively. Pretreatment with CTX 2 mg / mouse does not affect the generation of BALB / c nude mice at 4-5 weeks of age Leukemia model.