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为了探讨乙型肝炎(HB)的遗传传递,我们对乙型肝炎患者(HBP)38个家系82个个体检测了血清游离型和外周血单核细胞内整合型 HBV DNA U5样序列片段和单链 DNA 构象多态性等实验指标。发现男患者及其发病后出生子女 U5样序列检出率均显著高于其发病前出生子女(P<0.05);U5样序列检出率在 HBP 和其发病后出生子女呈一致性增高;HBP 与其发病后出生子女可具有完全一致的单链泳动变位结果。在正常人群基因组内无 HBV DNA U5样序列的同源序列。本研究结果为 HB的遗传传递学说提供了分子遗传学证据。
To investigate the genetic transmission of Hepatitis B (HB), 82 samples from 38 families of Hepatitis B patients (HBPs) were tested for seroconversion and integration of HBV U5-like sequences in peripheral blood mononuclear cells and single-stranded DNA conformation polymorphism and other experimental indicators. It was found that the detection rate of U5-like sequences in male patients and their post-birth children were significantly higher than those before birth (P <0.05). The detection rate of U5-like was consistently increased in HBP and their post-birth children. HBP And its incidence of children after birth can have exactly the same single-chain motile displacement results. There is no HBV DNA U5-like sequence homology in the normal human genome. The results of this study provide molecular genetic evidence for HB’s theory of genetic transmission.