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目的:与NDV疫苗株LaSota对比研究一株NDV D817株对肝癌细胞高效特异性的杀伤效应和作用机制,进一步筛选NDV溶瘤毒株。方法:用MTT法对比病毒对三株传代肝癌细胞株SMMC-7721、Bel-7404和HepG-2及一株正常肝细胞株HL-7702的杀伤效应,并用TUNNL法及透射电镜观察病毒诱导肿瘤细胞发生凋亡作用。结果:NDV D817株对肝癌细胞株SMMC-7721、Bel-7404和HepG-2杀伤效应高达80%,显著高于疫苗LaSota株(P<0.01),而对人正常肝细胞HL-7702无明显影响;病毒在肝癌细胞中明显复制增殖,对细胞的杀伤活性与病毒作用剂量和病毒作用时间成正比;NDV D817株有效诱导肝癌细胞发生凋亡。结论:NDV D817株有效诱导肝癌细胞发生凋亡,对SMMC-7721、Bel-7404和HepG-2细胞具有高效杀伤性,而对正常肝细胞HL-7702未见明显影响。推测为溶瘤株。
OBJECTIVE: To compare the killing effect and mechanism of NDV D817 against NDV vaccine strain LaSota on hepatocellular carcinoma cells, and further screen NDV oncolytic strains. Methods: The killing effects of three viruses on the three hepatoma cell lines SMMC-7721, Bel-7404 and HepG-2 and one normal hepatocyte cell line HL-7702 were compared by MTT assay. The virus-induced tumor cells were observed by TUNNL method and transmission electron microscope Apoptosis occurs. Results: The killing effect of NDV D817 on hepatocellular carcinoma cell lines SMMC-7721, Bel-7404 and HepG-2 was as high as 80%, significantly higher than that of vaccine LaSota (P <0.01), but not on human normal hepatocytes HL-7702 ; The virus significantly replicates and proliferates in hepatoma cells, and the killing activity to the cells is proportional to the dose of the virus and the time of the virus. The NDV D817 strain effectively induces the apoptosis of the hepatoma cells. CONCLUSION: NDV D817 strain can effectively induce apoptosis of hepatocellular carcinoma cells and has high cytotoxicity against SMMC-7721, Bel-7404 and HepG-2 cells, but no significant effect on normal liver cells HL-7702. Speculated as oncolytic strain.