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目的建立同时测定檀香叶中牡荆苷与异牡荆苷含量测定方法,为其资源利用等提供依据。方法采用Agilent TC-C18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-0.5%冰醋酸溶液(40∶60),检测波长为338nm,柱温为30℃,流速为1 mL·min-1。结果牡荆苷的线性范围为7.625~488.0μg·mL-1;高、中、低剂量回收率分别为96.38%、97.23%、97.96%,RSD为1.29%~1.83%。异牡荆苷的线性范围为8.047~515.0μg·mL-1,高、中、低剂量回收率分别为97.81%、96.13%、97.98%,RSD为1.31%~1.81%。三批样品中牡荆苷与异牡荆苷的含量为1.13%~1.25%和1.20%~1.46%。结论檀香叶中牡荆苷与异牡荆苷含量较高,所建立的含量测定方法简便、准确,可用于檀香叶的质量控制。
Objective To establish a method for the simultaneous determination of vitexin and isovitexin in sandalwood leaves, and to provide basis for its utilization. Methods The mobile phase was methanol-0.5% glacial acetic acid (40:60) with an Agilent TC-C18 column (4.6 mm × 250 mm, 5 μm) with a detection wavelength of 338 nm and a column temperature of 30 ℃ with a flow rate of 1 mL · min-1. Results The linear range of vitexin was 7.625 ~ 488.0μg · mL-1. The recoveries of high, medium and low doses were 96.38%, 97.23% and 97.96%, respectively, and the RSDs were 1.29% -1.83%. The linear range of iso-vitexin was 8.047-515.0 μg · mL-1, and the recoveries of high, medium and low doses were 97.81%, 96.13% and 97.98%, respectively. The RSDs ranged from 1.31% to 1.81%. The contents of vitexin and isovitexin in three batches of samples were 1.13% -1.25% and 1.20% -1.46%. Conclusions The content of vitexin and isovitexin in sandalwood leaves is high, and the established method for determining the content of vitexin is simple and accurate, which can be used for the quality control of sandalwood leaves.