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The s gene of five prevalent virulent strains and one C-strain of Hog Cholera Virus were amplified by reverse transcription (RT). The amplified fragments were cloned and sequenced. Comparison and analysis were made on the nucleotide sequence and the amino acid sequence of five prevalent virulent strains of HCV was deduced. The nucleotide homology was from 91% to 98%; the homology of amino acid was from 94% to 98%. The nucleotide homology of C-strain virus from cell cultured and five prevalent virulent strains of HCV was 83% to 84% .The amino acid homology was from 89% to 91%.