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目的:建立大鼠膈神经和迷走神经放电以及呼吸和心电图同步记录的技术,为研究各指标的动态变化和各指标之间的关系以及多种因素对其的影响奠定基础。方法:用Wistar大鼠10只。20%乌拉坦腹腔注射后,仰卧固定;颈部正中切口,暴露气管并插管,分离左膈神经和右迷走神经,记录其放电;用
OBJECTIVE: To establish the technique of synchronous recording of phrenic nerve, vagal nerve discharge and respiration and electrocardiogram in rats, which lays the foundation for studying the dynamic changes of each index and the relationship among various indexes and the influence of various factors on it. Methods: 10 Wistar rats were used. 20% of urethane intraperitoneal injection, the supine fixed; neck incision, exposed trachea and intubation, the left phrenic nerve and the right vagus nerve separation, recording the discharge; with