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目的研究凝血酶敏感蛋白-1(TSP-1)对胆囊癌细胞GBC-SD凋亡的体外诱导作用及其可能的作用机制。方法采用流式细胞术检测TSP-1及其受体CD36,CD47作用后HCCLM3的凋亡率,通过逆转录聚合酶链反应(RT-PCR)Caspase-3 mRNA表达的变化。结果TSP-1组凋亡率(10.56±0.63)%显著高于对照组(3.17±0.33)%和CD47阻断组(4.05±0.27)%(P<0.01)。CD36阻断组(8.49±0.37)%高于对照组或CD47阻断组,低于TSP-1组,差异有统计学意义(P<0.01)。TSP-1组Caspase3 mRNA的表达TSP-1组(0.575±0.026)和CD36阻断组(0.583±0.023)显著高于对照组(0.336±0.031)和CD47阻断组(0.412±0.017),差异有统计学意义(P<0.01)。结论TSP-1在体外可诱导胆囊癌细胞GBC-SD的凋亡,TSP-1与受体CD47结合后上调Caspase3的表达可能是作用途径之一。
Objective To investigate the in vitro induction of thrombospondin-1 (TSP-1) on GBC-SD apoptosis in gallbladder carcinoma cells and its possible mechanism. Methods The apoptosis rate of HCCLM3 and the expression of Caspase-3 mRNA by reverse transcription-polymerase chain reaction (RT-PCR) were detected by flow cytometry after TSP-1 and its receptors CD36 and CD47. Results The apoptosis rate in TSP-1 group (10.56 ± 0.63)% was significantly higher than that in control group (3.17 ± 0.33)% and CD47 block group (4.05 ± 0.27)% (P <0.01). CD36 blockade group (8.49 ± 0.37)% higher than the control group or CD47 block group, lower than the TSP-1 group, the difference was statistically significant (P <0.01). The expression of Caspase3 mRNA in TSP-1 group (0.575 ± 0.026) and CD36 group (0.583 ± 0.023) was significantly higher than that in control group (0.336 ± 0.031) and CD47 block group (0.412 ± 0.017) Statistical significance (P <0.01). Conclusion TSP-1 can induce the apoptosis of gallbladder carcinoma cells GBC-SD in vitro. Upregulating the expression of Caspase3 after TSP-1 binds to receptor CD47 may be one of the mechanisms of action.