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目的对四川人群的13个短串联重复(short tandem repeat,STR)基因座进行遗传多态性调查。方法 310份血样采自四川地区无血缘关系个体。Chelex法提取DNA,PCR复合扩增,自动基因分析仪电泳收集电泳结果数据,基因分型软件进行样本基因型分型。结果 13个STR基因座的基因型分布符合Hardy-Weinberg平衡。累计非父排除率和累计个人识别率为0.999991728和>0.999999999。结论上述13个STR基因座的累计非父排除率和累计个人识别率较高,适合作为四川人群的遗传标记,用于法医学亲权鉴定和个体识别等领域的研究。
Objective To investigate the genetic polymorphisms of 13 short tandem repeat (STR) loci in Sichuan population. Methods 310 blood samples were collected from unrelated individuals in Sichuan Province. Chelex DNA extraction, PCR amplification, automatic gene analyzer electrophoresis data collection electrophoresis, genotyping software sample genotyping. Results The distribution of 13 STR loci was in accordance with Hardy-Weinberg equilibrium. The cumulative non-parent exclusion rate and cumulative personal recognition rate are 0.999991728 and> 0.999999999. Conclusion The above 13 STR loci have a high non-father exclusion rate and a high cumulative personal recognition rate, and are suitable as genetic markers in Sichuan population for the study of forensic paternity testing and individual identification.