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目的:利用多元回归方法构建预测生物剂量模型,探讨多基因表达组合作为生物剂量估算方法的可行性。方法:体外照射人外周血,照射剂量为0 Gy-12 Gy,照后培养0 h-12 h,实时定量RT-PCR方法检测TNFSF4、CCL2、CCL7的照后表达变化,建立预测生物剂量模型。结果:照后0-12 h,TNFSF4、CCL2和CCL7随着培养时间的延长三者的相对表达量增加(P<0.001,P=0.005,P<0.001);在0-12Gy剂量范围内,各剂量组的CCL7相对表达量差异有统计学意义(P=0.009),而TNFSF4和CCL2相对表达量差异没有统计学意义(P=0.254,P=0.059,P=0.137)。照后12 h,TNFSF4,CCL2和CCL7纳入模型,建立的预测生物剂量模型R2高于单基因估算剂量的R2(0.757>0.482)。结论:多基因表达组合作为生物剂量估算方法具有一定的可行性。
OBJECTIVE: To construct a predictive bio-dose model by multiple regression and to explore the feasibility of multi-gene expression as a biological dose estimation method. Methods: Peripheral blood was irradiated at a dose of 0 Gy-12 Gy in vitro and then cultured for 0 h-12 h. Real-time quantitative RT-PCR was used to detect the expression of TNFSF4, CCL2 and CCL7, and a predictive bio dose model was established. RESULTS: At 0-12 h after irradiation, the relative expression of TNFSF4, CCL2 and CCL7 increased with the prolongation of culture time (P <0.001, P = 0.005, P <0.001) The relative expression level of CCL7 in the dose group was significantly different (P = 0.009), while the relative expression level of TNFSF4 and CCL2 was not statistically different (P = 0.254, P = 0.059, P = 0.137). At 12 h after irradiation, TNFSF4, CCL2 and CCL7 were included in the model, and the predicted bio-dose model, R2, was higher than the estimated R2 (0.757> 0.482) for the single gene. Conclusion: The combination of multi-gene expression as a biological dose estimation method has a certain feasibility.