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土壤丛枝菌根(Arbuscular mycorrhizal,AM)真菌具有维持植被稳定性和多样性的重要作用.为了解空心莲子草入侵过程中与AM真菌的相互作用,采用脂肪酸甲酯(FAMEs)和基于18S rDNA的PCR-DGGE方法对湖北咸宁、仙桃和武汉三地空心莲子草(Alternanthera philoxeroides)入侵前后土壤AM真菌的生物量和群落结构进行了比较分析.结果发现,基于FAMEs表征的AM真菌生物量在空心莲子草入侵后变化不大,仅仙桃地区显著增加(P<0.05).尽管DGGE图谱显示咸宁、仙桃和武汉三地AM真菌优势类群的群落结构均发生了明显演替,但群落间物种组成差异的多样性指数βWH在2.55-2.73之间、群落多样性指数H′在1.22-1.38之间,变化均不大.结果表明,空心莲子草入侵所形成的植物更替明显改变了AM真菌优势类群的结构,但对AM真菌的生物量和多样性影响很小.
Arbuscular mycorrhizal (AM) fungi have an important role in maintaining the stability and diversity of vegetation.In order to understand the interaction between AM fungi and AM fungi, FAMEs and 18S rDNA (PCR-DGGE) method was used to analyze the biomass and community structure of AM fungi before and after invasion of Alternanthera philoxeroides in Xianning, Xiantao, and Wuhan of Hubei Province.The results showed that the biomass of AM fungi based on FAMEs was significantly higher in hollow The results showed that there was no significant change in the lotus seedlings after invader, but only in Xiantao region (P <0.05). Although the DGGE maps showed that the community structures of dominant AM fungi in Xianning, Xiantao and Wuhan were significantly changed, The results showed that alternation of Alternanthera philoxeroides plants significantly changed the dominant groups of AM fungi Structure, but little effect on AM fungi biomass and diversity.