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目的:观察太子参醇提物(EPHPH)对自然衰老模型大鼠听性脑干反应(ABR),耳蜗组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、一氧化氮合酶(NOS)及分型一氧化氮合酶(iNOS)的影响,探讨其对老年性聋的作用及其机制。方法:自然衰老模型大鼠分别灌胃给予太子参2.16、4.32及8.64g·kg-1·d-1的醇提物,每日1次,共6个月,分别测定自然衰老模型大鼠ABR,耳蜗组织中MDA、SOD、NOS及iNOS。结果:自然衰老模型大鼠ABR阈值明显升高,并伴有耳蜗组织中MDA明显升高,SOD、NOS及iNOS活力明显下降。相关性分析表明,ABR阈值升高与耳蜗组织中MDA升高呈正相关;与SOD、NOS及iNOS活力明显下降均呈负相关。自然衰老模型大鼠灌胃太子参2.16、4.32及8.64g·kg-1·d-1的醇提物,能不同程度对抗ABR阈值升高及耳蜗组织中MDA升高,其量效关系均呈负相关;能不同程度抑制SOD、NOS和iNOS活力降低,其量效关系均呈正相关。结论:EPHPH可减轻老年性聋程度,其机制可能与清除耳蜗组织中氧自由基,提高SOD及NOS活力有关。
OBJECTIVE: To observe the auditory brainstem response (ABR) in the rat model of natural aging and the malondialdehyde (MDA), superoxide dismutase (SOD), and nitric oxide synthase in the cochlea The effects of nitric oxide synthase (NOS) and nitric oxide synthase (iNOS) on the role and mechanism of iNOS were investigated. METHODS: Natural aging rats were given intragastric administration of 2.16, 4.32 and 8.64 g·kg-1·d-1 ethanol extracts, respectively, once daily for 6 months to determine the natural aging model rat ABR. , MDA, SOD, NOS and iNOS in the cochlea tissue. RESULTS: The threshold value of ABR in natural aging rats was significantly elevated, and MDA content in cochlear tissues was significantly increased, and the activities of SOD, NOS, and iNOS were significantly decreased. The correlation analysis showed that the increase of ABR threshold was positively correlated with the increase of MDA in cochlear tissue, and negatively correlated with the significant decrease of SOD, NOS and iNOS activity. The alcohol extracts from 2.16, 4.32 and 8.64g·kg-1·d-1 of Taizishen fed by natural aging model rats could resist the ABR threshold elevation and increase the MDA content in the cochlea tissue in varying degrees. The dose-effect relationship was presented. Negative correlation; can inhibit SOD, NOS and iNOS activity decrease in varying degrees, and their dose-effect relationships are positively correlated. Conclusion: EPHPH can reduce the degree of senile delirium. The mechanism may be related to the removal of oxygen free radicals in the cochlear tissue and increase of SOD and NOS activity.