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目的检测大肠癌患者 p16基因启动子CpG区甲基化的改变 ,探讨其与大肠癌发生、发展和临床资料的关系。 方法采用甲基化特异PCR技术 (MSP法 )研究 5 8例大肠癌标本 p16基因甲基化改变 ,分析临床病理资料。 结果 2 5例标本 (4 3 .1% )呈 p16CpG区甲基化阳性 ;DukesC、D期病人p16CpG区甲基化发生率 (75 % )明显高于DukesA、B期病人 (13.3% )。 结论 p16启动子区甲基化导致p16抑癌基因失活参与了大肠癌的发生和发展 ;p16甲基化可能可以作为估计大肠癌病人预后的一个指标
Objective To detect the changes in the methylation of the p16 promoter CpG region in patients with colorectal cancer and to explore its relationship with the occurrence, development and clinical data of colorectal cancer. Methods Methylation-specific PCR (MSP) was used to study the methylation changes of p16 gene in 58 cases of colorectal carcinoma and analyze the clinicopathological data. RESULTS: Twenty-five specimens (43.1%) were positive for methylation in p16CpG region; the incidence of methylation in p16CpG region (75%) was significantly higher in Dukes C and D patients than in Dukes A and B patients (13.3%). Conclusion The methylation of p16 promoter leads to the inactivation of p16 tumor suppressor gene involved in the occurrence and development of colorectal cancer; p16 methylation may be used as an index to estimate the prognosis of colorectal cancer patients.