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[目的]利用近红外漫反射光谱建立小麦抗旱性鉴定体系。[方法]2006-2007年有针对性地选择36份抗旱性不同的小麦材料,通过利用小麦抗旱性鉴定评价技术体系规范(GBT21127-2007)进行抗旱性鉴定分类分级,同时利用FOSSNIRSystems5000型近红外谷物品质分析仪(全光谱分析仪),对收获后的小麦籽粒样品进行光谱采集,并建立预测回归方程。[结果]经过建库和验证,初步得到判别方程。经比较,水分胁迫下的方程参数RSQ(检验决定系数)=0.8460、I-VR(交互检验决定系数)=0.7818,品种的抗旱性和光谱特征的一致性较好,小麦籽粒近红外慢反射光谱与小麦的抗旱性具有较好的相关性。[结论]在水分胁迫的情况下,通过近红外漫反射光谱技术扫描小麦籽粒样品建立一套简单、快速、无损的小麦抗旱性鉴定体系是可行的。
[Objective] The research aimed to establish identification system of drought resistance in wheat by using near-infrared diffuse reflectance spectroscopy. [Method] Thirty-six wheat varieties with different drought resistance were selected from 2006 to 2007, and their drought resistance was identified and classified by GBT21127-2007. At the same time, FOSSNIRSystems5000 near-infrared grain Quality analyzer (full spectrum analyzer), the harvested wheat grain samples were collected by spectroscopy and the regression equation was established. [Results] After establishing the database and verifying, the discriminant equation was obtained initially. Compared with RSQ (test determination coefficient) = 0.8460 and I-VR (interaction test determination coefficient) = 0.7818 under water stress, the drought resistance and spectral characteristics of varieties are in good agreement with each other, And wheat drought resistance has a good correlation. [Conclusion] Under the condition of water stress, it is feasible to establish a simple, rapid and non-destructive identification system of drought resistance in wheat by scanning wheat grain samples by near-infrared diffuse reflectance spectroscopy.