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目的调查石家庄地区慢性胃病患者感染幽门螺杆菌(H·pylori)的细胞毒素相关基因(cagA)及空泡毒素基因(vacA)亚型的流行情况;探讨H·pylori cagA、vacA亚型与胃肠疾病的关系。方法自石家庄地区慢性胃病患者胃黏膜中分离得到55株H·pylori,抽提各菌株的总DNA,采用特定引物对各菌株cagA3′端、vacA信号序列(s)及中间序列(m)进行PCR检测。结果H·pylori cagA的阳性者占89·1%(49/55),其在慢性胃炎和消化性溃疡中的阳性率差异无显著性(χ2=0·376,P=0·540)。H·pylori vacA基因亚型有s1a/m2、s1a/m1b-m2、s1a/m1b三种组合,各亚型所占比例分别为90·4%(47/52)、5·8%(3/52)、3·8%(2/52),其中最常见的vacA亚型s1a/m2在慢性胃炎与消化性溃疡中的阳性率差异无显著性(χ2=0,P=1·000)结论石家庄地区慢性胃病患者感染的幽门螺杆菌以cagA+、vacA s1a/m2亚型占优势,未发现H·pylori cagA及vacA亚型与特定胃肠疾病间存在显著相关性。
Objective To investigate the prevalence of cytotoxin related gene (cagA) and vacuolar vacuole (vacA) in Helicobacter pylori infection in patients with chronic gastritis in Shijiazhuang area. To investigate the prevalence of H · pylori cagA, vacA subtype and gastrointestinal The relationship between the disease. Methods 55 H · pylori strains were isolated from gastric mucosa of patients with chronic gastritis in Shijiazhuang area and the total DNA of each strain was extracted. PCR was performed on cagA 3 ’end, vacA signal sequence (s) and middle sequence (m) of each strain using specific primers Detection. Results The positive rate of H. pylori cagA was 89.1% (49/55). There was no significant difference in the positive rates of chronic gastritis and peptic ulcer (χ2 = 0.376, P = 0.504). The genotypes of vacA gene of H. pylori were s1a / m2, s1a / m1b-m2 and s1a / m1b. The proportion of each subtype was 90.4% (47/52) and 5.8% (3 / 52) and 3.8% (2/52) respectively. There was no significant difference in the positive rates of the most common vacA subtype s1a / m2 between chronic gastritis and peptic ulcer (χ2 = 0, P = 1000) Helicobacter pylori infection in patients with chronic gastritis in Shijiazhuang was predominant in the cagA + and vacA s1a / m2 subtypes. No significant correlation was found between the cagA and vacA subtypes of H. pylori and the specific gastrointestinal diseases.