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本文以不同浓度钙离子螯合剂 EGTA、钙通道阻断剂异博啶 (Vp)和 Ca M拮抗剂三氟啦嗪 (TFP)处理小麦幼苗 ,对 PEG胁迫下根和叶片 ABA和 Ca M含量的变化进行了测定。结果表明 :EGTA、Vp和 TFP处理皆能促进根和叶片 ABA含量的提高。PEG胁迫下不同浓度 EGTA和 Vp处理后 Ca M含量也不同程度地提高 ,TFP处理可减弱PEG对 Ca M含量升高的促进作用。从 ABA和 Ca M最大峰值出现的时间上看 ,Ca M都滞后于 ABA。上述结果暗示 :Ca2 + / Ca M可能参与干旱信号 ABA的信息传递过程 ,而 ABA合成过程与胞质 Ca2 + 浓度有关。
In this paper, we treated wheat seedlings with different concentrations of calcium ion chelator EGTA, calcium channel blocker (VP) and CaM antagonist trifluoperazine (TFP). The contents of ABA and Ca M in roots and leaves under PEG stress Changes were measured. The results showed that EGTA, Vp and TFP all promoted ABA content in roots and leaves. The content of CaM in PEG-treated EGTA and Vp-treated groups also increased to different extents. TFP treatment decreased the effect of PEG on the increase of CaM content. Ca M lags behind ABA from the time when the largest peak of ABA and Ca M occurs. The above results suggest that Ca2 + / CaM may be involved in the signal transduction of drought signal ABA, while ABA synthesis is related to the concentration of cytoplasmic Ca2 +.