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选取不同代次的ＬＲ１毒株在Ｖｅｒｏ细胞上传代适应，不同天数连续动态观察细胞病变情况，同时用免疫荧光法和ＥＬＩＳＡ进行抗原检测，收毒前细胞反复冻融及超声波破碎，细胞破碎前后用ＥＬＩＳＡ检测抗原含量，半微量空斑法检测病毒滴度。结果证明：ＬＲ１株病毒能在Ｖｅｒｏ细胞上产生细胞病变，病变程度与其毒力明显相关；ＬＲ１株病毒在Ｖｅｒｏ细胞上繁殖的最佳收毒时间为１１天左右；病毒释放性较差，冻融和超声波破碎细胞能明显提高其抗原含量和病毒滴度
LR1 strains of different generations were selected and passaged on Vero cells for adaption. The cells were continuously and dynamically observed for different days. Antigen was detected by immunofluorescence and ELISA. The cells were repeatedly frozen and thawed and sonicated before harvesting. Antigen content was detected by ELISA and virus titer by semi-micro plaque assay. The results showed that: LR1 strain of virus can produce cytopathic effect in Vero cells, the degree of lesion was significantly related to its virulence; LR1 strain of Vero cells in the optimal propagation time of about 11 days; virus release is poor, freeze-thaw And sonicated cells can significantly increase its antigen content and virus titer