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目的观察补肾益髓(BSYS)方及其拆方补肾(BS)和化痰活血(HTHX)方对实验性自身免疫性脑脊髓炎(EAE)小鼠脑和脊髓中β-淀粉样前体蛋白(β-APP)和微管相关蛋白-2(MAP-2)的作用。方法 56只雌性C57BL/6小鼠随机分为正常对照组(NC)、模型组(MO)、醋酸泼尼松组(PA)、梓醇组(CA)、补肾益髓组(BSYS)、补肾组(BS)和化痰活血组(HTHX),每组8只。对照药给予PA(6 mg/kg),CA(40 mg/kg);BSYS、BS和HTHX组分别以3.02 g,1.44 g和1.57 g生药/kg给药。NC及MO组以等量蒸馏水代替。每日1次,连续灌胃40 d。小鼠发病第25日(急性期)和第40日(缓解期)取脑和脊髓,采用免疫荧光(IF)法检测β-APP表达,免疫组化(IHC)法检测MAP-2的表达。结果发病第25日和第40日,MO组小鼠脑β-APP表达较NC组显著上调(P<0.001),而MAP-2表达则较NC组明显下调(P<0.01或P<0.001),与MO组比较,各治疗组(PA、CA、BSYS、BS和HTHX组)均可明显下调β-APP蛋白表达(P<0.05或P<0.01),上调MAP-2表达(P<0.05或P<0.01)。其中发病第25日小鼠脑β-APP表达PA组效果优于CA、BS及HTHX组(P<0.05)。各组小鼠脊髓内β-APP、MAP-2表达变化趋势与脑相似。结论 BSYS方及其拆方均能减轻轴突损伤及促进其修复,尤以BSYS全方更为显著。
Objective To observe the effects of Bushen Yisui (BSYS) and its constituents tonifying the kidney (BS) and phlegm and activating blood (HTHX) on the expression of β-amyloid precursor protein in the brain and spinal cord of experimental autoimmune encephalomyelitis (EAE) (β-APP) and microtubule-associated protein-2 (MAP-2). Methods 56 female C57BL / 6 mice were randomly divided into normal control group (NC), model group (MO), prednisone acetate group (PA), catalpol group (CA), Bushen Yisui group (BSYS) Group (BS) and phlegm and blood activating group (HTHX), 8 in each group. The control drug was given at 6 mg / kg and CA at 40 mg / kg. The BSYS, BS and HTHX groups were administered at 3.02 g, 1.44 g and 1.57 g crude drug / kg, respectively. NC and MO group with the same amount of distilled water instead. 1 day, continuous gavage 40 d. The brain and spinal cord were taken on the 25th day (acute phase) and the 40th day (remission stage) in mice. The expression of β-APP was detected by immunofluorescence (IF) and the expression of MAP-2 by immunohistochemistry (IHC) Results On the 25th day and the 40th day, the expression of β-APP in MO group was significantly higher than that in NC group (P <0.001), while the MAP-2 expression was significantly lower than that in NC group (P <0.01 or P <0.001) Compared with the MO group, all the treatment groups (PA, CA, BSYS, BS and HTHX groups) could significantly downregulate the expression of β-APP protein (P <0.05 or P <0.01) P <0.01). The incidence of β-APP on the 25th day in mice was better than that in CA, BS and HTHX groups (P <0.05). The changes of β-APP and MAP-2 in spinal cord of mice in each group were similar to those in brain. Conclusion BSYS prescription and its disassembly can both reduce axonal injury and promote its repair, especially in the whole of BSYS.