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应用免疫组化ＬＳＡＢ法及流式细胞分析技术（ＦＣＭ），对１２例早期胃癌，１５例胃癌前病变（８例胃粘膜中～重度不典型增生，７例轻度不典型增生）及６例正常胃粘膜增殖细胞核抗原（ＰＣＮＡ）的表达及细胞核ＤＮＡ含量进行了测定。着重探讨了胃粘膜癌变过程中ＰＣＮＡ的表达及ＤＮＡ含量变化的意义，试图为胃癌的早期诊断提供客观依据。结果表明：ＰＣＮＡ标记指数（ＬＩ）随胃粘膜病变程度的加重呈递增趋势。中～重度不典型增生与早期胃癌之间无明显差异（Ｐ＞０．０５）；ＤＮＡ非整倍体检出率比较，早期胃癌明显高于胃粘膜轻度不典型增生组（Ｐ＜０．０１）。
Using immunohistochemistry LSAB method and flow cytometric analysis (FCM), 12 cases of early gastric cancer, 15 cases of gastric precancerous lesions (8 cases of moderate to severe dysplasia in gastric mucosa, 7 cases of mild dysplasia) and 6 cases Normal gastric mucosal proliferating cell nuclear antigen (PCNA) expression and nuclear DNA content were measured. The significance of PCNA expression and DNA content changes in gastric carcinogenesis was emphasized, and it was attempted to provide an objective basis for the early diagnosis of gastric cancer. The results showed that the PCNA labeling index (LI) was increasing with the severity of gastric mucosal lesions. There was no significant difference between moderate-severe atypical hyperplasia and early gastric cancer (P>0.05). The detection rate of DNA aneuploidy was significantly higher in early gastric cancer than in mild dysplasia of gastric mucosa (P<0.01). ).