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目的:黄芪、灯盏花素对实验性糖尿病(DM)大鼠肾脏内皮素-1(ET-1)、转化生长因子β1(TGF-β1)水平变化的影响。方法:SD大鼠45只随机分为3组:正常对照组(NC)、DM对照组和DM治疗组(DT)。DT组用黄芪7.0 g/kg和灯盏花素1.2 g/kg,水煎后灌喂,1次/d,共6周。观察大鼠血糖、胰岛素、总胆固醇(TC)、三酰甘油(TG)、肌酐清除率(Ccr)、尿白蛋白排泄率(UAE)、尿β2微球蛋白排泄率(β2-MG)等水平变化;以放射免疫法检测血浆、尿液中ET-1水平;以ELISA法检测血清、尿液TGF-β1水平。结果:糖尿病大鼠的血糖、胰岛素、TC和TG升高;治疗组血糖、血胰岛素、TC和TG显著降低。糖尿病模型组血、尿中ET-1、TGF-β1均显著高于正常对照组;治疗组血、尿中ET-1、TGF-β1水平及Ccr、UAE、β2-MG显著降低。结论:黄芪、灯盏花素对糖尿病大鼠的肾脏具有保护作用,而抑制糖尿病大鼠ET-1、TGF-β1水平可能是其重要作用机制。
Objective: To investigate the effects of Astragalus membranaceus and breviscapine on the changes of renal endothelin-1 (ET-1) and transforming growth factor-β1 (TGF-β1) in experimental diabetic rats. Methods: 45 SD rats were randomly divided into 3 groups: normal control group (NC), DM control group and DM treatment group (DT). DT group with Astragalus 7.0 g / kg and breviscapine 1.2 g / kg, after decoction fed, 1 / d, a total of 6 weeks. The levels of blood glucose, insulin, total cholesterol (TC), triglyceride (TG), creatinine clearance (Ccr), urinary albumin excretion (UAE) and urinary β2 microglobulin excretion (β2-MG) The levels of ET-1 in plasma and urine were detected by radioimmunoassay. The levels of TGF-β1 in serum and urine were detected by ELISA. Results: The blood glucose, insulin, TC and TG increased in diabetic rats. The blood glucose, blood insulin, TC and TG in the treatment group were significantly decreased. The levels of ET-1 and TGF-β1 in the blood and urine of diabetic model group were significantly higher than those in the normal control group. The levels of ET-1, TGF-β1 and the levels of Ccr, UAE and β2-MG in the blood and urine of the untreated group were significantly decreased. Conclusion: Astragalus and breviscapine have a protective effect on the kidney of diabetic rats, and the inhibition of ET-1 and TGF-β1 in diabetic rats may play an important role.