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目的建立高良姜醇提物中2个二苯基庚烷类化合物的测定方法。方法采用GC法,色谱柱为Cp Sil 5CB(30 m×0.25 mm,0.25μm)石英毛细管柱;检测器:FID;柱温:初始温度80℃,保持2 min,以20℃/min升温到300℃,保持45 min。结果 1-苯基-7-(3’-甲氧基-4’-羟基)苯基-5-醇-3-庚酮在0.2～1.0 mg/mL(r=0.998 8)、1,7-二苯基-5-醇-3-庚酮在0.2～1.0 mg/mL(r=0.997 8)有较好的线性关系;平均回收率分别为99.6%、100.6%。结论本方法可靠、专属性强,可作为高良姜醇提物中2个二苯基庚烷类化合物的测定方法。
OBJECTIVE To establish a method for the determination of 2 diphenylheptanes in Alpinia officinarum alcohol extract. Methods GC method was used. The column was a silica gel column with a capacity of 30 × 0.25 mm × 0.25 mm. The detector was FID. The column temperature was 80 ℃ and kept for 2 min. The temperature was raised to 300 at 20 ℃ / min ℃, hold for 45 min. Results 1-phenyl-7- (3’-methoxy-4’-hydroxy) phenyl-5- Diphenyl-5-ol-3-heptanone showed a good linearity between 0.2 and 1.0 mg / mL (r = 0.997 8). The average recoveries were 99.6% and 100.6%, respectively. Conclusion The method is reliable and specific and can be used as a method for the determination of 2 diphenylheptanes in Alpinia officinarum alcohol extract.