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目的 应用多抗原肽 (MAP)研究丙型肝炎病毒包膜糖蛋白高变区 1(HVR1)的抗原性。方法 根据已经获得的HCV BJ株E2 /NS1区氨基酸序列 ,参照国内外学者所报道的HCVHVR1序列及抗原性参数 ,设计并合成含HCVHVR1390— 411aa序列 2 2个氨基酸的线性表位多肽 (以LP表示 )及MAP(对称 8分枝 ) ,分别以LP和MAP免疫Balb/C小鼠及家兔 ,比较其免疫原性。结果 MAP免疫原性明显强于LP ,抗体滴度 1∶40 96 0 ,而LP免疫组为 1∶12 80。结论 MAP的构型优势能明显提高HCVHVR1合成多肽抗原的免疫原性 ,提示MAP可用于HCV分子疫苗的设计。
Objective To study the antigenicity of hepatitis C virus envelope glycoprotein hypervariable region 1 (HVR1) by using multi-antigen peptide (MAP). Methods Based on the amino acid sequence of E2 / NS1 of HCV BJ strain and the HCVHVR1 sequences and antigenicity parameters reported by domestic and foreign scholars, a linear epitope peptide of 22 amino acids with HCVHVR1390- 411aa sequence was designed and synthesized ) And MAP (symmetrical 8-branched). Balb / C mice and rabbits were immunized with LP and MAP respectively to compare their immunogenicity. Results The immunogenicity of MAP was significantly stronger than that of LP. The titer of antibody was 1:40 96 0, while that of LP group was 1:12 80. Conclusion The conformational advantages of MAP can significantly improve the immunogenicity of HCVHVR1 synthetic peptide antigen, suggesting that MAP can be used in the design of HCV molecular vaccine.