论文部分内容阅读
目的:建立气相色谱法测定藿香祛暑软胶囊中百秋李醇、丁香酚2组分的含量。方法:采用CP-WAX52CB毛细管柱,程序升温:初始温度100℃,以3℃·min~(-1)的速率升温至150℃,再以10℃·min~(-1)的速率升温至230℃;载气为氮气,恒压方式,压力为25 Pai;检测器FID。结果:百秋李醇在0.031～0.154μg(r=0.996 6)、丁香酚在0.075～0.374μg(r=0.999 7)的范围内进样量与峰面积比呈良好的线性关系,百秋李醇平均回收率为98.31%(RSD=1.12%)、丁香酚平均回收率98.95%(RSD=0.59%)。结论:该方法快速、准确、简便。
Objective: To establish a method for the determination of the contents of berberine and eugenol 2 in Huoxiangqu Shu soft capsule by gas chromatography. Methods: CP-WAX52CB capillary column was used to program the temperature rising: the initial temperature was 100 ℃, the temperature was increased to 150 ℃ at the rate of 3 ℃ · min ~ (-1), and the temperature was raised to 230 ℃ at the rate of 10 ℃ · min ~ (-1) ℃; carrier gas is nitrogen, constant pressure mode, the pressure of 25 Pai; detector FID. Results: There was a good linear relationship between the injection volume and the peak area ratio of P. chinensis in the range of 0.031 ~ 0.154μg (r = 0.996 6) and eugenol in the range of 0.075 ~ 0.374μg (r = 0.999 7) The average recovery of alcohol was 98.31% (RSD = 1.12%). The average recovery of eugenol was 98.95% (RSD = 0.59%). Conclusion: This method is fast, accurate and easy.