论文部分内容阅读
贵州省不同地区大豆上的8个细菌菌株,通过大豆致病性试验,染色应反,形态、培养性状的观察,以及生理生化和血清学反应的测定,8个菌株在大豆叶片上引起褐色小病斑。革兰氏染色阴性,直杆状,大小0.5～0.8×1.0～3.5微米,极生鞭毛1～4根。在单一碳源的基础培养基中生长,绝对好气的荧光假单胞杆菌。在牛肉汁蛋白胨培养基上菌落园形,乳白色,光滑,微隆,边缘整齐。精氨酸双解酶和氧化酶阴性。不液化明胶,不水解淀粉,从葡萄糖和蔗糖产酸,不产生吲哚,硝酸盐不还原。石蕊牛奶变碱,产果聚糖,利用甘露醇,D-山梨醇,肌醇,甜菜碱,酒石酸盐和乳酸盐。与GS4抗血清的凝集反应一致。由此认为,贵州省大豆上的细菌叶斑病是由Pseudomonas syringae pv.glyclinea(Coerper 1919)Young.Dye ＆ Wilkie1978引起。
Eight strains of soybeans in different regions of Guizhou Province were tested for pathogenicity of soybean, anti-stained, morphological and cultural traits, and physiological, biochemical and serological tests. Eight strains caused brownish-yellow on soybean leaves Lesions. Gram stain negative, straight rod, the size of 0.5 ~ 0.8 × 1.0 ~ 3.5 microns, polar flagella 1 ~ 4. Pseudomonas fluorescens, which grows absolutely on the base medium of a single carbon source. Colonies in beef juice peptone colonies, milky white, smooth, micro-Long, neat edges. Arginine diol and oxidase negative. Does not liquefy gelatin, does not hydrolyze starch, acid from glucose and sucrose, does not produce indole, nitrate is not reduced. Litmus milk base, fructan, mannitol, D-sorbitol, inositol, betaine, tartrate and lactate. Consistent with the agglutination reaction of GS4 antiserum. Therefore, the bacterial leaf spot on soybean in Guizhou Province was caused by Pseudomonas syringae pv. Glyclinea (Coerper 1919) Young. Dye & Wilkie 1978.