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目的对比微波、紫外线和环氧乙烷对Silagum硅橡胶义齿软衬材料的消毒效果。方法将高温消毒后的软衬硅橡胶试件染菌(白色念珠菌),分别进行不同时间的紫外线、微波、环氧乙烷消毒,消毒后的试件用无菌生理盐水冲洗,无菌玻璃平皿收集冲洗液,接种环将冲洗液接种于沙氏琼脂平板培养基,37℃恒温培养24～48 h观察,菌落计数,评价消毒效果。结果环氧乙烷(EO浓度600 mg/L)消毒60 min灭菌率为100%;紫外线(功率40 W、波长253.7 nm、距离1 m)照射40 min可杀灭99%的白色念珠菌、50 min可完全杀灭;微波(800 W100%)照射1 min灭菌率为93%,照射2 min灭菌率为100%。结论三种消毒方法都具有消毒效果,且消毒时间越长消毒效果越好;环氧乙烷、紫外线、微波消毒口腔硅橡胶义齿软衬材料可与高温高压灭菌达到同样的效果,而且方便、有效,是良好的软衬材料的消毒方法。
Objective To compare the disinfection effects of microwave, ultraviolet and ethylene oxide on Silagum silicone rubber denture soft lining materials. Methods After the high temperature disinfection of soft lining silicon rubber specimens (Candida albicans), respectively, at different times of UV, microwave, ethylene oxide disinfection, disinfection of the specimen with sterile saline irrigation, sterile glass Plates were collected rinse, inoculation loop inoculation rinse liquid in sand agar plate medium, incubated at 37 ℃ for 24-48 h observation, colony count, evaluation of disinfection. Results The sterilization rate of ethylene oxide (EO 600 mg / L) for 60 min was 100%, and 99% of Candida albicans was killed by ultraviolet (power 40 W, wavelength 253.7 nm, distance 1 m) 50 min can completely kill; microwave (800 W100%) irradiation 1 min sterilization rate was 93%, irradiation 2 min sterilization rate was 100%. Conclusion The three disinfection methods have the effect of disinfection, and the longer the disinfection time, the better the disinfection effect; ethylene oxide, ultraviolet, microwave disinfection oral silicone rubber denture soft lining material can be sterilized with high temperature and pressure to achieve the same effect, and convenient, Effective, is a good method of disinfection of soft lining material.